Chandigarh, Thursday, March 25, 1999 |
DNA fingerprinting by Dinesh Kumar Forensic science is one of the fascinating branches of science. Movies and television serials are made showing how a diligent detective was able to piece together a crime in seemingly impossible circumstances. In real life, however, detectives hardly have the time to solve cases, let alone go in for rigorous research. Many of their techniques are not taken as conclusive evidence. The only positive leads available to forensic science is through fingerprints: since each person has a unique finger-pattern, a criminal can theoretically be nabbed. Science, society and
scientist Response
|
DNA fingerprinting Forensic science is one of the fascinating branches of science. Movies and television serials are made showing how a diligent detective was able to piece together a crime in seemingly impossible circumstances. In real life, however, detectives hardly have the time to solve cases, let alone go in for rigorous research. Many of their techniques are not taken as conclusive evidence. The only positive leads available to forensic science is through fingerprints: since each person has a unique finger-pattern, a criminal can theoretically be nabbed. Other techniques have not had unequivocal success. But one method is increasingly being used the world over in solving crime cases: DNA fingerprinting. It has been used in thousands of cases since it was used in 1986. In that case, it showed that a person who had confessed to committing a rape had actually not done it and the analysis helped in catching the real culprit. Even in cases where there are no clues, DNA can be collected from minute samples at the scene of the crime which no criminal can wipe out completely. DNA are strands of genetic material found in tissues and all parts of the body such as blood, skin hair and so on. It is unique for each human being, except in identical twins. If it is picked up from a scene of crime, it can help solve crimes by matching the DNA of suspects. It is thus a foolproof method. The problem was often in obtaining enough quantities of tissue from the scene of the crime which could help establish the DNA profile of the criminal. In the 1980s, a new technique was invented: the polymerase chain reaction (PCR) technique. This allows DNA analysis to be performed on minute amounts of tissue. It amplifies small quantities of DNA and replicates the molecules. In this technique, first the DNA molecule is exposed to high temperatures, which makes it come apart. An enzyme called polymerase is added to the mixture so that the double helix cannot come together, but forms a new strand. The DNA is thus doubled in the laboratory and the process can be repeated till enough is obtained for proper analysis. The DNA then works like a signature, since the variation among people is concentrated at certain places in their DNA. Another signature is found in DNA microsatellites, which are short segments in which the sequence of the genetic latters repeats itself. Since the DNA works as a signature for each human being, laboratory results can produce an accurate profile of the DNA of a person. Thus, taking pieces of saliva, hair and semen from the scene of the crime is essential and will become invaluable clues. The next step is to do the DNA fingerprinting of suspects in order to match the results obtained. In cases of rape, getting a victim to a DNA expert would almost certainly establish proof of the involvement of the culprit, since other evidence is hard to come by or is not enough to convict. If DNA databases are maintained of the population, all that needs to be done is to do a computer match of the DNA obtained to zero in on the culprit. Since building a DNA database for millions of people is a huge task, this may not be possible right as yet. However, obtaining DNA samples of suspects is not a difficult task and crime fighting can be greatly helped through this technique. There have been controversial cases no doubt, but the DNA technique is accepted widely as accurate. How can our country benefit from the technique? The first step is to create DNA testing facilities in hospitals and forensic laboratories. Researchers must be trained in these techniques. As the incidence of crime is increasing in the country, it is time we started fighting it with modern scientific methods. DNA testing should thus be more widespread than it is now. The modern forensic
detective may not be as romantic as Sherlock Holmes, with
a magnifying glass in his hand. He may not be the
colourful characters we see on television screens either.
He may well be a white coat clad scientist in a
laboratory who can identify DNA signatures. |
Science, society and scientist To publicise or not to publicise what they are doing is a Shakespearean dilemma that confronts scientists. Left to himself, every serious scientist would perhaps be happy to keep himself closeted in his laboratory or observatory. After all, a purist's view is that publishing diminishes the quality of work through overclaims and mistaken analogies, etc and even of the scientist through descending to the common man's level. Surprisingly, and fortunately, one of the country's topmost scientists, Jayant Vishnu Narlikar, holds a diametrically opposite view. An interaction at Pune recently provided one an opportunity to discuss the subject with him. In jest, he explains his "publicise or perish" theory by saying that publicising one's work is necessary not only because a scientist's credibility rests on peer review (which demands publicising) but also because research grants are at stake! But on a more serious note he elucidates the issue through a triangle. The point to note is that each side of it has two-way arrows, signifying two-way interaction. Take the A-B (science-scientist) side first. Science opens a new door only at the right time, as it happened last century when quantum mechanics and relativity came up after thermodynamics. Science has always attempted to hit at wrong approaches through new findings. The scientist on his part takes up new challenges of nature to advance the frontiers of science. He uses new ideas for practical purposes. In science-society interface also, there is two-way communication. Science through its technological inventions controls society whereas society controls science for reasons of survival, politics and commerce. But perhaps the most important is the scientist-society relationship. The former is dependent on society for continuing his or her work while the latter requires the scientist to work in a certain way for its compulsions. That makes it imperative for a scientist to publicise his work. A scientist owes it to society in return for its support, according to Professor Narlikar. Once the necessity of publicising is accepted, the next task is to identify and eliminate the problems of publicising at the right level. The first is how to convey genuine excitement about an advancement, invention or discovery, without succumbing to hype. A glaring example is the almost-monthly announcements about the discovery of a black hole. After all, a black hole can never be really discovered. Then comes the question of "descending" to the level of the common man. Professor Narlikar calls it "ascending" instead. The way he sees it, most scientists dig deep to find the truth. They have to "ascend" to the ground level to enlighten the layman about what they have found. The trouble is that not all scientists with something to communicate are good communicators. They can reveal their mind to those who are on the same wavelength but are at sea when trying to present it to the common man. Even those who are good are not bothered about communicating, because they are over-eager to avoid public misconceptions. They cannot be faulted for that either. Besides ensuring that they do not misuse the general public's lack of expertise on scientific matters to inflate their own achievements, scientists have to avoid overkill or backlash because aggressive support for a factual idea, which does not have widespread support, can be counterproductive. Even when a scientist
practises such transparency, there are times when vested
interests try to silence him. That is what hurts the
most. Even as luminous a mind as Narlikar has had to
suffer this ignominy. Some years ago, when he, along with
some other prominent scientists, proposed an alternative
to the Big Bang theory, the US-based referee rejected it
as "outlandish". That is the cross that many a
scientist has to bear. |
I have carefully gone through the contents of the letter of Mr G.S. Dhillon published in the Science Tribune of Feb 11. I stand by the details shown by me in my article as the same have been worked out after taking into account the point raised by Mr Dhillon regarding changes in concrete. Mr Dhillon has raised another point that the common user may not be able to verify the type of steel as Tor-40 or Tor-55 without getting it tested. This is not correct. A common user can easily identify any type of steel without getting it tested. There are distinctive marks on the steel bars which dont seem to be in the knowledge of Mr Dhillon. While sending the article, I had desired to write another small piece showing guidelines for buying steel. Now that Mr Dhillon has raised this point, I find it my duty to explain these guidelines: 1. A plain mild steel bar can be easily distinguished from a deformed bar. Please note that the technical name of deformed bars is HYSD bars i.e. High Yield Strength Deformed bars while their popular name is Tor steel as its authorised licencees are Tor-Allies Engineering Services Ltd. 2. Tor-40 steel can be easily identified by a close examination of deformed bars. These bars carry the word Tor engraved on the steel bars at every one metre distance. 3. Tor-50 steel bars carry an additional mark 50 or 0 in between every two Tor marks. That is how Tor-40 and Tor-50 can be distinguished. 4. Sometimes Tor-50 bars carry a mark * in between every two Tor marks. This star is very easy to locate. 5. Tor-55 steel bars carry an additional mark 55 in between every two Tor marks. 6. All Tor-50 steel bars are painted green for a length of 4 inches at both ends. 7. All Tor-55 bars are painted blue for a length of 4 inches at ends. 8. Tor-40 bars are not at all painted at ends. 9. All defective steel bars are painted red at both ends by the manufacturers. 10. Tor steel licencees supply a test-certificate for any quantity of steel supplied by them. This certificate is so produced that half of it is embossed in readable form while half of it is printed. This is done to avoid misuse of the certificate or to avoid taking out its photocopies. |
H |